Should I Submit Mouse Serum or Plasma to Rules-Based Medicine for Rodent Multi-Analyte Profiling?
Introduction
When discussing projects with our customers, they often ask, "Should we collect serum or plasma in our mouse study?" In this case study, we used data generated by our Rodent MAP antigen panel version 1.5 to compare the mean values for each of the 59 analytes measured. These data were generated with 3,502 mouse serum samples and 2,946 mouse plasma samples submitted to RBM for testing (386,880 data points). These data therefore represented many different strains of mice across many different experimental regimens. The purpose was simply to answer the question: "Is my analyte of interest better represented in serum or plasma?" This may serve as a guide for your own experimental design.
RodentMAP Screening of Three Commonly Used Mouse Strains
Comparison of Males and Females at Three Ages for C57, 129 and FVB Mouse Strains Using the Rodent MAP.
Introduction:
Many investigators that utilize mice for research and development purposes have inquired about the "normal ranges" for the analytes measured in Rules-Based Medicine's Rodent Multi-Analyte Profile (MAP). To this end, Charles River Laboratories and Rules-Based Medicine used the RodentMAP to biochemically phenotype plasma samples from three commonly used mouse strains.
Should I Use Human Serum or Plasma for my HumanMAP Study?
Comparison of Serum to Three Plasma Anticoagulant Samples (EDTA, Citrate and Heparin) using the Human MAP.
Abstract:
Blood is commonly processed into serum or plasma for the measurement of analytes depending upon their stability and reproducibility in each matrix. With the development of multiplexing, it has become critical to understand the behavior of many different analytes in the four most common sample types.
The ob/ob mouse: A Case Study
Rules-Based Medicine and Charles River Laboratories compared the phenotypes of the classic ob/ob homozygote mouse with both its wild-type form and with the ob/ob mutant treated with an anti-obesity drug. The protein expression patterns and ensuing results are presented using the powerful OmniViz data mining software.
Case Studies
Should I Submit Mouse Serum or Plasma to Rules-Based Medicine for Rodent Multi-Analyte Profiling?
RodentMAP Screening of Three Commonly Used Mouse Strains
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